Massively parallel rRNA gene sequencing exacerbates the potential for biased community diversity comparisons due to variable library sizes.

@article{Gihring2012MassivelyPR,
  title={Massively parallel rRNA gene sequencing exacerbates the potential for biased community diversity comparisons due to variable library sizes.},
  author={Thomas M. Gihring and Stefan J Green and Christopher W. Schadt},
  journal={Environmental microbiology},
  year={2012},
  volume={14 2},
  pages={285-90}
}
Technologies for massively parallel sequencing are revolutionizing microbial ecology and are vastly increasing the scale of ribosomal RNA (rRNA) gene studies. Although pyrosequencing has increased the breadth and depth of possible rRNA gene sampling, one drawback is that the number of reads obtained per sample is difficult to control. Pyrosequencing libraries typically vary widely in the number of sequences per sample, even within individual studies, and there is a need to revisit the behaviour… CONTINUE READING
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