Mass spectrometry of the fifth nucleoside: a review of the identification of pseudouridine in nucleic acids.

@article{Durairaj2008MassSO,
  title={Mass spectrometry of the fifth nucleoside: a review of the identification of pseudouridine in nucleic acids.},
  author={Anita Durairaj and Patrick A. Limbach},
  journal={Analytica chimica acta},
  year={2008},
  volume={623 2},
  pages={117-25}
}
Pseudouridine, the so-called fifth nucleoside due to its ubiquitous presence in ribonucleic acids (RNAs), remains among the most challenging modified nucleosides to characterize. As an isomer of the major nucleoside uridine, pseudouridine cannot be detected by standard reverse-transcriptase-based DNA sequencing or RNase mapping approaches. Thus, over the… CONTINUE READING