Mass spectrometry: from proteomics to metabolomics and lipidomics.

  title={Mass spectrometry: from proteomics to metabolomics and lipidomics.},
  author={William J. Griffiths and Yuqin Wang},
  journal={Chemical Society reviews},
  volume={38 7},
Mass spectrometry (MS) in combination with liquid chromatography (LC), i.e. LC-MS, is the key analytical technique on which the emerging "omics" technologies of proteomics, metabolomics and lipidomics are based. It provides both structural and quantitative data and can be used in a "global" or "targeted" manner allowing on the one hand the identification of thousands of proteins from a tissue, or on the other the detection of biologically active metabolites at levels of a few parts-per-billion… 

On the future of “omics”: lipidomics

The underlying fundamentals of different lipidomic experimental approaches including the “shotgun” and “top-down” global approaches, and the more targeted liquid chromatography – or gas Chromatography – mass spectrometry approaches are examined.

The Role of Mass Spectrometry in the “Omics” Era

The main advances in the MS technologies are focused on, influencing genomics, transcriptomics, proteomics, lipidomics and metabolomics fields, up to the most recent MS applications to meta-omic studies.

Proteomics, Metabolomics and Lipidomics in Reproductive Biotechnologies: The MS Solutions

This manuscript describes recent advances of MS in the proteomics, metabolomics and lipidomics for biological sciences, and points out the relevant contributions that MS is likely to bring to fundamental and applied research in human and animal embryo biotechnologies.

Applications of mass spectrometry to lipids and membranes.

Deuterium exchange mass spectrometry is being used to investigate the association of lipids and membranes with proteins and enzymes, and imaging mass Spectrometry (IMS) is being applied to the in situ analysis oflipids in tissues.

Mass spectrometry for the evaluation of cardiovascular diseases based on proteomics and lipidomics.

This paper will review the different mass spectrometry-based proteomic and lipidomic strategies applied in cardiovascular diseases, especially atherosclerosis, with particular attention given to recent developments and the role of bioinformatics in data treatment.

Overview of mass spectrometry-based metabolomics: opportunities and challenges.

This chapter provides a glimpse at the current status of the mass spectrometry-based metabolomics field highlighting the opportunities and challenges.

Mass spectrometry based environmental metabolomics: a primer and review

This review provides the first comprehensive overview of the use and capabilities of MS within environmental metabolomics and introduces environmental scientists to the range of MS approaches used in metabolomics to highlight the breadth and diversity of environmental and ecological research conducted.

Targeted metabolomics for biomarker discovery.

This Review highlights important technical prerequisites as well as recent developments in metabolomics and metabolomics data analysis with special emphasis on their utility in biomarker identification and qualification, aswell as targeted metabolomics by employing high-throughput mass spectrometry.



Shotgun lipidomics: electrospray ionization mass spectrometric analysis and quantitation of cellular lipidomes directly from crude extracts of biological samples.

This review will focus on the advances in ESI/MS, which have facilitated the development of shotgun lipidomics and the utility of intrasource separation as an enabling strategy for utilization of 2D mass spectrometry in Shotgun lipidomics of biological samples.

From exogenous to endogenous: the inevitable imprint of mass spectrometry in metabolomics.

The aims of developing mass spectrometry for metabolomics range from understanding basic biochemistry to biomarker discovery and the structural characterization of physiologically important metabolites.

Tandem mass spectrometry in the study of fatty acids, bile acids, and steroids.

The FAB and ES ionization techniques will be discussed in relation to MS/MS, and examples of their application in biochemical studies will be presented.

Liquid chromatography-mass spectrometry utilizing multi-stage fragmentation for the identification of oxysterolss⃞ Published, JLR Papers in Press, January 24, 2007.

A novel liquid chromatography (LC) multi-stage fragmentation mass spectrometry (MSn) methodology for the identification, with high sensitivity (low pg), of cholesterol metabolites in rat brain was described.

Mass spectrometry-based proteomics

The ability of mass spectrometry to identify and, increasingly, to precisely quantify thousands of proteins from complex samples can be expected to impact broadly on biology and medicine.

Top-down lipidomic screens by multivariate analysis of high-resolution survey mass spectra.

Direct profiling of total lipid extracts on a hybrid LTQ Orbitrap mass spectrometer by high-resolution survey spectra clusters species of 11 major lipid classes into 7 groups, which are distinguished

The Human Plasma Proteome

This work speculates on the reasons behind this large discrepancy between the expectations arising from proteomics and the realities of clinical diagnostics and suggests approaches by which protein-disease associations may be more effectively translated into diagnostic tools in the future.

An update on lipidomics: progress and application in biomarker and drug development.

  • Xianlin Han
  • Biology, Chemistry
    Current opinion in molecular therapeutics
  • 2007
An update of technical developments within the field of lipidomics from 2006 to the present is provided, along with a brief discussion of its applications in biomarker and drug development.