Mass spectrometric mapping of protein epitope structures of myocardial infarct markers myoglobin and troponin T.

@article{Macht1996MassSM,
  title={Mass spectrometric mapping of protein epitope structures of myocardial infarct markers myoglobin and troponin T.},
  author={Marcus Macht and Waltraud Fiedler and Konrad K{\"u}rzinger and Michael Przybylski},
  journal={Biochemistry},
  year={1996},
  volume={35 49},
  pages={15633-9}
}
Monoclonal antibodies are widely used analytical tools in biochemical research. The knowledge of their corresponding epitopes is of major interest. One possible approach for epitope characterization is the application of protein antigen proteolysis in combination with mass spectrometric peptide mapping analysis. Two complementary analytical strategies were applied: (a) limited proteolysis of antibody-bound antigen followed by removal of nonbound peptides and detachment of the antigenic peptides… CONTINUE READING