Mass spectrometric characterization of the glycosylation pattern of HIV-gp120 expressed in CHO cells.

@article{Zhu2000MassSC,
  title={Mass spectrometric characterization of the glycosylation pattern of HIV-gp120 expressed in CHO cells.},
  author={X G Zhu and Christoph H. Borchers and Rachelle J Bienstock and Kenneth B. Tomer},
  journal={Biochemistry},
  year={2000},
  volume={39 37},
  pages={11194-204}
}
An analytical approach is reported for the characterization of the specific glycans found on highly glycosylated proteins based on a combination of specific proteolysis and deglycosylation combined with two different mass spectrometric approaches, matrix-assisted laser desorption/ionization mass spectrometry, and nanoelectrospray mass spectrometry/tandem mass spectrometry using a hybrid quadrupole-time-of-flight tandem mass spectrometer. The high resolution and mass accuracy of the mass… CONTINUE READING

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