Mass spectrometric and peptide chip epitope mapping of rheumatoid arthritis autoantigen RA33.

@article{ElKased2009MassSA,
  title={Mass spectrometric and peptide chip epitope mapping of rheumatoid arthritis autoantigen RA33.},
  author={Reham F El-Kased and Cornelia Koy and T Deierling and Peter Lorenz and Zhong Qian and Ying Shiun Li and H. J. Thiesen and Michael O. Glocker},
  journal={European journal of mass spectrometry},
  year={2009},
  volume={15 6},
  pages={747-59}
}
The protein termed RA33 was determined to be one major autoantigen in rheumatoid arthritis (RA) patients and antiRA33 auto-antibodies were found to appear shortly after onset of RA. They are often detectable before a final diagnosis can be made in the clinic. The aim of our study is to characterise the epitope of a monoclonal antiRA33 antibody on recombinant RA33 using mass spectrometric epitope mapping. Recombinant RA33 has been subjected to BrCN cleavage and fragments were separated by sodium… CONTINUE READING