Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins.

@article{Zachariae1998MassSA,
  title={Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins.},
  author={W. Zachariae and Andrej Shevchenko and Paul D. Andrews and Rafal Ciosk and Marta G{\'a}lov{\'a} and Michael J R Stark and Matthias Mann and Kim Nasmyth},
  journal={Science},
  year={1998},
  volume={279 5354},
  pages={1216-9}
}
Entry into anaphase and exit from mitosis depend on a ubiquitin-protein ligase complex called the anaphase-promoting complex (APC) or cyclosome. At least 12 different subunits were detected in the purified particle from budding yeast, including the previously identified proteins Apc1p, Cdc16p, Cdc23p, Cdc26p, and Cdc27p. Five additional subunits purified in low nanogram amounts were identified by tandem mass spectrometric sequencing. Apc2p, Apc5p, and the RING-finger protein Apc11p are… CONTINUE READING
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