Mass spectrometric analysis of rat ovary and testis cytosolic glutathione S-transferases (GSTs): identification of a novel class-alpha GST, rGSTA6*, in rat testis.

Abstract

Cytosolic glutathione S-transferases (GSTs) from rat ovaries and testis were purified by a combination of GSH and S-hexylglutathione affinity chromatography. The isolated GSTs were subjected to reverse-phase HPLC, electrospray MS and N-terminal peptide sequencing analysis. The major GST isoenzymes expressed in ovaries are subunits A3, A4, M1, M2 and P1… (More)

Topics

Cite this paper

@article{Hsieh1997MassSA, title={Mass spectrometric analysis of rat ovary and testis cytosolic glutathione S-transferases (GSTs): identification of a novel class-alpha GST, rGSTA6*, in rat testis.}, author={C H Hsieh and Shan Pou Tsai and Hung I Yeh and T C Sheu and Ming F. Tam}, journal={The Biochemical journal}, year={1997}, volume={323 ( Pt 2)}, pages={503-10} }