Mass isolation and culture of rat kupffer cells

  title={Mass isolation and culture of rat kupffer cells},
  author={A C Munthe-Kaas and Trond Berg and Per O. Seglen and Rolf Seljelid},
  journal={The Journal of Experimental Medicine},
  pages={1 - 10}
Collagenase perfusion of the liver followed by pronase treatment of the cell suspension thus obtained gave a quantitative recovery of viable nonparenchymal liver cells (NPC). From these NPC, Kupffer (K) cells can be purified by attachment to tissue culture dishes. Tail vein injection of carbon 1-2 h before liver perfusion permitted stepwise calculation as well as visualization of carbon-containing K cells. When these K cells have been put into tissue culture medium with serum and incubated… CONTINUE READING
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  • Scientific Publications Ltd
  • 1970

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