Mass-Based Metabolomic Analysis of Lactobacillus sakei and Its Growth Media at Different Growth Phases.

@article{Lee2017MassBasedMA,
  title={Mass-Based Metabolomic Analysis of Lactobacillus sakei and Its Growth Media at Different Growth Phases.},
  author={Sang Bong Lee and Young Kyoung Rhee and Eun-Ji Gu and Dong-Wook Kim and Gwang-Ju Jang and Seonghwa Song and Jae-In Lee and Bo-Min Kim and Hyeon-Jeong Lee and Hee-Do Hong and Chang-Won Cho and Hyun-Jin Kim},
  journal={Journal of microbiology and biotechnology},
  year={2017},
  volume={27 5},
  pages={
          925-932
        }
}
Changes in the metabolite profiles of Lactobacillus sakei and its growth media, based on different culture times (0, 6, 12, and 24 h), were investigated using gas chromatography-mass spectrometry (MS) and liquid chromatography-MS with partial least squares discriminant analysis, in order to understand the growth characteristics of this organism. Cell and media samples of L. sakei were significantly separated on PLS-DA score plots. Cell and media metabolites, including sugars, amino acids, and… 
Antioxidant activity changes of exopolysaccharides with different carbon sources from Lactobacillus plantarum LPC-1 and its metabolomic analysis
TLDR
It is indicated that Lactobacillus strain, depending on the carbon source in the medium, could produce EPSs of different biological properties, and the metabolomic analysis findings provided the first omics view of cell growth and EPSs synthesis in L. plantarum, which would be a theoretical basis for further improving the production of EPSs.
Shotgun Metagenomics and Volatilome Profile of the Microbiota of Fermented Sausages
TLDR
An advance in the understanding of meat fermentation is presented by coupling DNA sequencing metagenomics and metabolomics approaches to describe the microbial function during this process by exploiting the connections between the microbiota, microbiome, and the release of volatile metabolites during ripening.
Metabolomic profile of milk fermented with Streptococcus thermophilus cocultured with Bifidobacterium animalis ssp. lactis, Lactiplantibacillus plantarum, or both during storage.
TLDR
The findings suggest that the profile of the metabolites characterizing fermented milk samples may depend on the starter cultures, and incorporation of probiotics may considerably influence the metabolomic activities of fermented milks.
Application of Liquid Chromatography-Mass Spectrometry-Based Metabolomics in Cell Culture, Drug Study, and Diseases
TLDR
Overall, this paper provides an objective description of the LC-MS-based metabolomics, and summarizes its application in cell culture, drug study, and diseases.
Lactobacillus sakei: A Starter for Sausage Fermentation, a Protective Culture for Meat Products
TLDR
The emerging exploration of the microbial ecology of meat products revealed the multiplicity of bacterial interactions L. sakei has to face and their various consequences on microbial quality and safety at the end of storage.
Antifungal activity and functional components of cell-free supernatant from Bacillus amyloliquefaciens LZN01 inhibit Fusarium oxysporum f. sp. niveum growth
TLDR
This study suggests that B. amyloliquefaciens LZN01 is a promising biological agent against Fon as its CFS exerts antifungal activity by deforming conidial structures and damaging membranes and myriocin was one of the major functional components of the CFS.
Characterization of a new bacteriocin-like inhibitory peptide produced by Lactobacillus sakei B-RKM 0559.
TLDR
It was concluded that Sak-59 is a potential new bacteriocin with a characteristic activity spectrum, which can be useful in the food and feed industries.
Origin of Hypoglycemic Benefits of Probiotic-Fermented Carrot Pulp.
TLDR
The fermentation-induced improvement in diabetes control from fermented carrot pulp probably arises from its water-soluble polysaccharide WSP, which had stronger hydroxyl radical scavenging activity in vitro and showed better hypoglycemic effects than WSP from non-fermented carrot pulps in animal experiments.
...
1
2
...

References

SHOWING 1-10 OF 23 REFERENCES
Metabolic responses to Lactobacillus plantarum contamination or bacteriophage treatment in Saccharomyces cerevisiae using a GC–MS-based metabolomics approach
TLDR
Investigation of the intracellular biochemical changes in S. cerevisiae cells that were elicited by L. plantarum contamination or bacteriophage treatment found unique metabolite profiles originating from different groups were unique and could be distinguished with the aid of principal component analysis.
Optimization of the quenching method for metabolomics analysis of Lactobacillus bulgaricus
TLDR
A higher concentration of cold methanol quenching is suggested for L. bulgaricus metabolomics due to its decreasing metabolite leakage.
Relationships between arginine degradation, pH and survival in Lactobacillus sakei.
TLDR
It is shown that arginine degradation is responsible for an enhanced viability during the stationary phase when cells are grown under anaerobiosis and is necessary for the induction of the ADI pathway but in association with another environmental signal.
Comparative metabolomics of the diatom Skeletonema marinoi in different growth phases
TLDR
A rigorously validated protocol based on extraction, derivatisation and GC/MS for the analysis of diatom metabolomes is introduced and is in general suitable for the monitoring of microalgae and has the potential to be applied to complex plankton communities.
Adaptation of Lactobacillus sakei to meat: a new regulatory mechanism of ribose utilization?
TLDR
The gene cluster encoding a ribose transporter, ribose kinase and a regulator was cloned and sequenced and it was shown that the regulation of ribose utilization is not a transcriptional regulation.
Metabolic fingerprinting of Lactobacillus paracasei: a multi-criteria evaluation of methods for extraction of intracellular metabolites
TLDR
A superior coverage of FTM together with a high repeatability over nearly the whole range of GC-amenable compounds makes this the extraction method of choice for metabolic fingerprinting of L. paracasei.
The potential of lactic acid bacteria for the production of safe and wholesome food
TLDR
The wholesomeness of LAB can be extended to fields outside human nutrition, as they may act as probiotics in animal production or as plant protectives in agriculture and thus contribute to healthy raw materials for food production.
...
1
2
3
...