Mason-Pfizer monkey virus (MPMV) constitutive transport element (CTE) functions in a position-dependent manner.

@article{Rizvi1997MasonPfizerMV,
  title={Mason-Pfizer monkey virus (MPMV) constitutive transport element (CTE) functions in a position-dependent manner.},
  author={Tahir A. Rizvi and Russell D Schmidt and Kathy A Lew},
  journal={Virology},
  year={1997},
  volume={236 1},
  pages={
          118-29
        }
}
The Mason-Pfizer monkey virus (MPMV) constitutive transport element (CTE) is a cis-acting RNA element located in the 3' untranslated region (UTR) of the viral genome. The HIV-1 and SIV Rev/RRE regulatory system can be replaced with MPMV CTE (Bray et al., 1994; Zolotukhin et al., 1994; Rizvi et al., 1996a); similarly, CTE function can also be replaced by the HIV or SIV Rev/RRE regulatory system (Rizvi et al., 1996b; Ernst et al., 1997). In addition, we have shown that in the context of the SIV… 

Figures and Tables from this paper

Role of Purine-Rich Regions in Mason-Pfizer Monkey Virus (MPMV) Genomic RNA Packaging and Propagation

It is suggested that ssPurines and bpPurines play a redundant role in MPMV gRNA packaging, probably as Gag binding sites to facilitate gRNA capture and encapsidation to initiate the process ofMPMV genomic RNA (gRNA) packaging.

The upstream, direct repeat sequence of Prague A Rous sarcoma virus is deficient in mediating efficient Gag assembly and particle release.

The results suggest that unspliced RNA transport and Gag assembly functions may be mediated by different elements within the dr1 and that the Prague A upstream dr1 is defective in the latter but not the former function.

Stabilizing role of structural elements within the 5´ Untranslated Region (UTR) and gag sequences in Mason-Pfizer monkey virus (MPMV) genomic RNA packaging

Results reveal that sequences involved in forming these three stem loops do not play crucial roles at an individual level during MPMV gRNA packaging or propagation, and structure–function analysis indicates that the U5-Gag LRIs have a more important architectural role in stabilizing the higher order structure of the 5´ UTR than the threestem loops.

The 5′ RNA Terminus of Spleen Necrosis Virus Contains a Novel Posttranscriptional Control Element That Facilitates Human Immunodeficiency Virus Rev/RRE-Independent Gag Production

Analysis of SNV sequences reveals that the SNV 5′ RNA terminus contains a unique cis-acting posttranscriptional control element that interacts with hypothetical cellular Rev-like proteins to facilitate HIV RNA transport and efficient translation.

A structural role for the PHP domain in E. coli DNA polymerase III A structural role for the PHP domain in E. coli DNA polymerase III Let us know how access to this document benefits you.

The mechanism by which two gammaretroviruses, XMRV and MLV, synthesize the Gag polyprotein (Pr65 Gag ) from full-length, unspliced mRNA was investigated here and results are consistent with pol- encoded RNA acting in cis to promote Pr65Gag translation efficiency.

Gammaretroviral pol sequences act in cis to direct polysome loading and NXF 1 / NXT-dependent protein production by gag-encoded RNA

These experiments demonstrate that gammaretroviral pol sequences act in cis to recruit NXF1 and SRp20 to promote polysome loading of gag RNA and, thereby, license the synthesis of Pr65 from unspliced mRNA.

References

SHOWING 1-10 OF 58 REFERENCES

Role of Mason-Pfizer monkey virus (MPMV) constitutive transport element (CTE) in the propagation of MPMV vectors by genetic complementation using homologous/heterologous env genes.

It is concluded that CTE is important in the replication of MPMV and affects the nucleocytoplasmic transport and/or stability of viral messages similar to the Rev/RRE regulatory system of HIV-1 and SIV.

Rev/RRE-independent Mason-Pfizer monkey virus constitutive transport element-dependent propagation of SIVmac239 vectors using a single round of replication assay.

It is demonstrated that MPMV CTE is capable of substituting for SIV Rev and RRE functions, resulting in an attenuated ability to produce infectious virus.

A small element from the Mason-Pfizer monkey virus genome makes human immunodeficiency virus type 1 expression and replication Rev-independent.

It is shown that a cis-acting 219-nucleotide sequence from an unrelated "simple" retrovirus, Mason-Pfizer monkey virus (MPMV), enables Rev-independent HIV-1 replication and hypothesize that the MPMV element functions by interacting with a cellular factor that plays a role in mRNA transport analogous to that of the Rev protein.

The posttranscriptional control element of the simian retrovirus type 1 forms an extensive RNA secondary structure necessary for its function

Analysis of the molecular and structural requirements for function of a 240-nucleotide RNA element that can functionally replace posttranscriptional activation directed by Rev and the Rev-responsive element when inserted into a Rev- and RRE-deficient molecular clone of human immunodeficiency virus type 1, demonstrates the importance of the sequence and structure of the SRV-1 CTE for appropriate function.

Identification of an RNA sequence within an intracisternal-A particle element able to replace Rev-mediated posttranscriptional regulation of human immunodeficiency virus type 1

The results suggest that insertional mutagenesis can affect gene expression by providing a functional posttranscriptional control element in the form of a CTE or CTE of the type D simian retroviruses.

Continuous propagation of RRE(-) and Rev(-)RRE(-) human immunodeficiency virus type 1 molecular clones containing a cis-acting element of simian retrovirus type 1 in human peripheral blood lymphocytes

It is demonstrated that virus preparations produced after transfections of these SRV-1 element-containing molecular clones in human cells were infectious after cell-free transmission, that they replicated about 5 to 10 times less efficiently than wild-type virus, and that they were propagated continuously for more than 7 months in human peripheral blood mononuclear cells.

Differential effects of intronic and exonic locations of the human immunodeficiency virus type 1 Rev-responsive element.

It is demonstrated that the location of the RRE can have differential effects on the fate of HIV-1 RNAs by allowing the cytoplasmic accumulation of the spliced env mRNA, which lacked the R RE.

Avian retroviral RNA element promotes unspliced RNA accumulation in the cytoplasm

A cis-acting element in the 3' untranslated region of Rous sarcoma virus (RSV) RNA was found to promote Rev-independent expression of human immunodeficiency virus type 1 Gag proteins.

Relative roles of signals upstream of AAUAAA and promoter proximity in regulation of human immunodeficiency virus type 1 mRNA 3' end formation.

It is proposed that upstream elements play major roles in regulating poly(A) site choice and in controlling the subsequent fate of polyadenylated RNA in HIV-1 provirus through stabilizing nuclear transcripts and directly stimulating 3' end formation.
...