Marrow stromal fibroblastic cell cultivation in vitro on decellularized bone marrow extracellular matrix.


The in vitro biocompatibility of decellularized bone marrow extracellular matrix was evaluated. Following a freeze-thaw cycle, sectioned discs of fresh frozen rat metaphyseal bone were sequentially incubated in solutions of hypertonic, then hypotonic Ringer's solution, followed by deoxycholic acid, then DNAase I. The adequacy of decellularization of marrow… (More)
DOI: 10.1016/j.yexmp.2009.09.007


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