Marrow Stromal Cells as Stem Cells for Nonhematopoietic Tissues

@inproceedings{Prockop1997MarrowSC,
  title={Marrow Stromal Cells as Stem Cells for Nonhematopoietic Tissues},
  author={D. J. Prockop},
  booktitle={Science},
  year={1997}
}
  • D. Prockop
  • Published in Science 4 April 1997
  • Biology
Marrow stromal cells can be isolated from other cells in marrow by their tendency to adhere to tissue culture plastic. The cells have many of the characteristics of stem cells for tissues that can roughly be defined as mesenchymal, because they can be differentiated in culture into osteoblasts, chondrocytes, adipocytes, and even myoblasts. Therefore, marrow stromal cells present an intriguing model for examining the differentiation of stem cells. Also, they have several characteristics that… 
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  • 1999
TLDR
The composition, ontogeny, and function in physiological as well as pathological conditions of stroma are discussed, which includes, among other cell types, macrophages, fibroblasts, adipocytes, and endothelial cells.
Multilineage potential of adult human mesenchymal stem cells.
TLDR
Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
Mesenchymal stem cells: heading into the clinic
TLDR
The current understanding of bone marrow stroma and MSCs is reviewed and their potential therapeutic role in the setting of hematopoietic stem cell transplantation is reviewed.
Isolation of a Highly Clonogenic and Multipotential Subfraction of Adult Stem Cells from Bone Marrow Stroma
TLDR
A standardized assay by light scattering is developed to measure the content of rapidly self‐renewing cells (RS cells) in preparations of MSCs and enables us to isolate RS cells that were up to 90% clonogenic and that generated single cell–derived colonies that differentiated into either mineralizing cells or adipocytes with appropriate additions to the medium.
Mesenchymal cell contributions to the stem cell niche.
Marrow Stromal Mesenchymal Stem Cells
TLDR
This chapter summarizes the origins, isolation, characterization, cell cycle properties, and differentiation potential of bone marrow stromal mesenchymal stem cells (MSCs).
Plasticity of Bone Marrow–Derived Stem Cells
TLDR
The literature that lends credibility to the theory that highly plastic BMCs have a role in maintenance and repair of nonhematopoietic tissue is reviewed and the possible mechanisms by which this may occur are discussed.
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