Marker fusion tagging, a new method for production of chromosomally encoded fusion proteins.

@article{Lai2010MarkerFT,
  title={Marker fusion tagging, a new method for production of chromosomally encoded fusion proteins.},
  author={Julian U-Ming Lai and Seng kah Ng and Fang Liu and Rajesh Narhari Patkar and Yanfen Lu and Jing Ru Chan and Angayarkanni Suresh and Naweed I Naqvi and Gregory Jedd},
  journal={Eukaryotic cell},
  year={2010},
  volume={9 5},
  pages={
          827-30
        }
}
A new gene-tagging method (marker fusion tagging [MFT]) is demonstrated for Neurospora crassa and Magnaporthe oryzae. Translational fusions between the hygromycin B resistance gene and various markers are inserted into genes of interest by homologous recombination to produce chromosomally encoded fusion proteins. This method can produce tags at any position and create deletion alleles that maintain N- and C-terminal sequences. We show the utility of MFT by producing enhanced green fluorescent… CONTINUE READING
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