Marker-free plasmids for biotechnological applications - implications and perspectives.

@article{Oliveira2013MarkerfreePF,
  title={Marker-free plasmids for biotechnological applications - implications and perspectives.},
  author={Pedro H Oliveira and Juergen Mairhofer},
  journal={Trends in biotechnology},
  year={2013},
  volume={31 9},
  pages={539-47}
}
Nonviral gene therapy and DNA vaccines have become the first promising approaches to treat, cure, or ultimately prevent disease by providing genetic information encoded on a plasmid. Since 1989, more than 1800 clinical trials have been approved worldwide, and approximately 20% of them are using plasmid DNA (pDNA) as a vector system. Although much safer than viral approaches, DNA vectors generally do encode antibiotic resistance genes in the plasmid backbone. These antibiotic resistance markers… CONTINUE READING