• Corpus ID: 81892370

Marker-assisted pyramiding of leaf rust resistance genes Lr24 and Lr28 in wheat (Triticum aestivum)

@article{Chhuneja2011MarkerassistedPO,
  title={Marker-assisted pyramiding of leaf rust resistance genes Lr24 and Lr28 in wheat (Triticum aestivum)},
  author={Parveen Chhuneja and Yogesh Vikal and Satinder Kaur and Rippy Singh and Shruti Juneja and Navtej Singh Bains and Onika Berry and Achla Sharma and S. K. Gupta and Ashwini Marotirao Charpe and Kumble Vinod Prabhu and Harcharan Singh Dhaliwal},
  journal={Indian Journal of Agricultural Sciences},
  year={2011},
  volume={81}
}
A study was undertaken to pyramid two effective leaf rust resistance genes Lr24 and Lr28 in ‘PBW 343’ wheat (Triticum aestivum L. emend. Fiori & Paol.) background, a ruling variety of North Western Plains Zone using marker assisted selection. ‘PBW343’ near isogenic lines with Lr24 and Lr28 developed through backcross breeding, were intercrossed and the marker assisted selection was conducted in the F 1 to F4 segregating generations using SCAR markers S73 719 and S421 570 linked to Lr24 and Lr28… 

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References

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TLDR
The SCAR markers linked to leaf rust resistance genes Lr24 and Lr28 were used to validate the identity and to confirm the transfer of these genes in PBW 343 background and Reconstitution of PBW343 traits was observed in most of the near isogenic lines (NILs).

Adult plant response of near-isogenic lines and stocks of wheat carrying specific Lr genes against leaf rust

TLDR
Near-isogenic lines and stocks of common wheat carrying specific Lr genes conferred a high degree of adult plant resistance (APR), while wheats carrying LrUb, Lr31 + Lr27 and Lr34 conditioned moderate susceptibility.

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TLDR
Although a reduction in dough development time was found to be associated with Lr28, it can still be used extensively in wheat breeding programmes.

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TLDR
Marker-trait associations are now known for a number of simple, but difficult-to-score traits, so that MAS has been found useful for improvement of several of these important economic traits.

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TLDR
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TLDR
This work integrated background analysis using mapped microsatellite markers with foreground selection to identify superior lines that combine useful genes from a non-Basmati BB resistance donor line IRBB55 with grain and cooking quality characteristics of the popular Basmati rice variety 'Pusa BasMati I' (PB 1) employing backcross pedigree strategy.

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TLDR
This work demonstrates the successful application of marker-assisted selection for targeted introgression of multiple resistance genes into a premium quality rice variety, Samba Mahsuri.