Marker and promoter effects on heterologous expression in Aspergillus nidulans

  title={Marker and promoter effects on heterologous expression in Aspergillus nidulans},
  author={David Lubertozzi and Jay D Keasling},
  journal={Applied Microbiology and Biotechnology},
To study the effects of selection marker, promoter type, and copy number on heterologous expression in Aspergillus nidulans, strains were constructed with single- and multicopy plasmid integrations bearing a reporter gene (lacZ) under the control of either an inducible (alcA) or constitutive (gpdA) promoter and one of three Aspergillus nutritional marker genes (argB, trpC, or niaD). β-Galactosidase activity in the transformants varied over three orders of magnitude, with the majority of levels… CONTINUE READING

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