Marked in vitro spontaneous secretion of IgA by human rib bone marrow mononuclear cells.

@article{Alley1982MarkedIV,
  title={Marked in vitro spontaneous secretion of IgA by human rib bone marrow mononuclear cells.},
  author={Charles D. Alley and Goeffrey S. Nash and Richard P Macdermott},
  journal={Journal of immunology},
  year={1982},
  volume={128 6},
  pages={2604-8}
}
Mononuclear cells (MNC) isolated from bone marrow curettaged from human ribs spontaneously produced significant amounts of Iga in 14-day in vitro culture. When culture supernatants were tested for each immunoglobulin isotype by radioimmunoassay at days 7, 14, 21, and 28, the peak of spontaneous IgA production was reached by day 14. Culturing marrow MNC in the presence of the polyclonal activator pokeweed mitogen (PWM) ablated this spontaneous IgA activity, and did not stimulate the production… CONTINUE READING