Marinopyrrole A target elucidation by acyl dye transfer.

  title={Marinopyrrole A target elucidation by acyl dye transfer.},
  author={Chambers C Hughes and Yu-Liang Yang and Wei-ting Liu and Pieter C. Dorrestein and James J La Clair and William Fenical},
  journal={Journal of the American Chemical Society},
  volume={131 34},
The targeting of marinopyrrole A to actin was identified using a fluorescent dye transfer strategy. The process began by appending a carboxylic acid terminal tag to a phenol in the natural product. The resulting probe was then studied in live cells to verify that it maintained activity comparable to marinopyrrole A. Two-color fluorescence microscopy confirmed that both unlabeled and labeled materials share comparable uptake and subcellular localization in HCT-116 cells. Subsequent… CONTINUE READING

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