Mariner mutagenesis of Brucella melitensis reveals genes with previously uncharacterized roles in virulence and survival

@article{Wu2006MarinerMO,
  title={Mariner mutagenesis of Brucella melitensis reveals genes with previously uncharacterized roles in virulence and survival},
  author={Qingmin Wu and Jianwu Pei and Carol Turse and Thomas A. Ficht},
  journal={BMC Microbiology},
  year={2006},
  volume={6},
  pages={102 - 102}
}
Random gene inactivation used to identify cellular functions associated with virulence and survival of Brucella spp has relied heavily upon the use of the transposon Tn5 that integrates at G/C base pairs. Transposons of the mariner family do not require species-specific host factors for efficient transposition, integrate nonspecifically at T/A base pairs, and, at a minimum, provide an alternative approach for gene discovery. In this study, plasmid vector pSC189, containing both the hyperactive… CONTINUE READING

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