Marine protist diversity in European coastal waters and sediments as revealed by high-throughput sequencing.

@article{Massana2015MarinePD,
  title={Marine protist diversity in European coastal waters and sediments as revealed by high-throughput sequencing.},
  author={Ramon Massana and Ang{\'e}lique Gobet and St{\'e}phane Audic and David Bass and Lucie Bittner and Christophe Boutte and Aur{\'e}lie Chambouvet and Richard Christen and Jean-Michel Claverie and Johan Decelle and John R. Dolan and Micah Dunthorn and Bente Edvardsen and Irene Forn and Dominik Forster and Laure Guillou and Olivier Jaillon and Wiebe H. C. F. Kooistra and Ramiro Logares and Fr{\'e}d{\'e}ric Mah{\'e} and Fabrice Not and Hiroyuki Ogata and Jan Pawlowski and Massimo C. Pernice and Ian Probert and Sarah Romac and Thomas A Richards and S{\'e}bastien Santini and Kamran Shalchian-Tabrizi and Raffaele Siano and Nathalie Simon and Thorsten Stoeck and Daniel Vaulot and Adriana Zingone and Colomban de Vargas},
  journal={Environmental microbiology},
  year={2015},
  volume={17 10},
  pages={4035-49}
}
Although protists are critical components of marine ecosystems, they are still poorly characterized. Here we analysed the taxonomic diversity of planktonic and benthic protist communities collected in six distant European coastal sites. Environmental deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) from three size fractions (pico-, nano- and micro/mesoplankton), as well as from dissolved DNA and surface sediments were used as templates for tag pyrosequencing of the V4 region of the 18S… CONTINUE READING
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