Mapping three classical isozyme loci in tetrahymena: meiotic linkage of EstA to the ChxA linkage group.

We demonstrate a reliable method for mapping conventional loci and obtaining meiotic linkage data for the ciliated protozoan Tetrahymena thermophila. By coupling nullisomic deletion mapping with meiotic linkage mapping, loci known to be located on a particular chromosome or chromosome arm can be tested for recombination. This approach has been used to map… CONTINUE READING