Mapping the urea channel through the rabbit Na(+)-glucose cotransporter SGLT1.

@article{PanayotovaHeiermann2001MappingTU,
  title={Mapping the urea channel through the rabbit Na(+)-glucose cotransporter SGLT1.},
  author={M Panayotova-Heiermann and Ernest M Wright},
  journal={The Journal of physiology},
  year={2001},
  volume={535 Pt 2},
  pages={419-25}
}
1. The rabbit Na(+)-glucose cotransporter rbSGLT1 and its carboxy-terminal part, C5, which contains transmembrane helices 10-14 of SGLT1 and functions as a low affinity glucose uniporter, were expressed as individual proteins in Xenopus oocytes. Transport of 55 microM urea, ethylene glycol, mannitol and alpha-methyl-D-glucopyranoside (alphaMDG) by control oocytes and by oocytes expressing SGLT1 and C5 was studied by uptake measurements of the 14C-labelled substrates. 2. There was a 5- to 6-fold… CONTINUE READING