Mapping the sites of localization of epithelial sodium channel (ENaC) and CFTR in segments of the mammalian epididymis

@article{Sharma2019MappingTS,
  title={Mapping the sites of localization of epithelial sodium channel (ENaC) and CFTR in segments of the mammalian epididymis},
  author={Sachin Sharma and Israel Hanukoglu},
  journal={Journal of Molecular Histology},
  year={2019},
  volume={50},
  pages={141-154}
}
The sperm produced in the seminiferous tubules pass through the rete testis, efferent ducts, and epididymis. The epididymis has three distinct regions known as caput, corpus, and cauda. The transit through the epididymis is an essential process in sperm maturation. The lumen of each epididymal region has a unique fluid composition regulated by many ion channels and transporters in the epithelial cells. The objective of this study was to map the sites of localization of ion channels ENaC and… 
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11 Citations
Background Citations
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Results Citations
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11 Citations

High‐resolution imaging of the actin cytoskeleton and epithelial sodium channel, CFTR, and aquaporin‐9 localization in the vas deferens

The localization of all three channels on the apical surface of the columnar epithelial cells provides clear evidence that these channels are involved concurrently in the regulation of fluid and electrolyte balance in the lumen of the vas deferens.

An atlas of human proximal epididymis reveals cell-specific functions and distinct roles for CFTR

A single cell atlas of the human proximal epididymis reveals key cell-specific functions, regional specialization, and two roles for CFTR, which is most abundant in surface epithelial cells in the efferent ducts and in rare clear Cells in the caput epidIDymis, suggesting region-specific functional properties.

Identification and classification of epithelial cells in nephron segments by actin cytoskeleton patterns

This study highlights the power of high‐resolution confocal microscopy for identifying specific cell types using the simple probe of F‐actin‐binding phalloidin to identify whole kidney sections by confocal microscopic imaging of fluorescent phalloids.

Development and Differentiation of Epididymal Epithelial Cells in Korean Native Black Goat

This study is the first to use immunofluorescence labeling and confocal imaging to characterize the differentiation of clear cells and basal cells in the goat epididymis, and could help the understanding of the regulatory mechanism that creates an optimal luminal environment for sperm maturation and storage in the epididykis.

Epithelial sodium channel (ENaC) (version 2019.4) in the IUPHAR/BPS Guide to Pharmacology Database

ENaC function is also essential for the clearance of respiratory airways, transport of germ cells, fertilization, implantation and cell migration, and evidence has been provided that rare mutations in ENaC are associated with female infertility.

The Distribution and Role of the CFTR Protein in the Intracellular Compartments

A summary of the present state of knowledge regarding CFTR localization and function in intracellular compartments, the physiological role of this localization and the consequences of protein dysfunction at cellular, epithelial and organ levels are provided.

Mechanosensitive Piezo1 channel in rat epididymal epithelial cells promotes transepithelial K+ secretion.

Importance of SLC26 Transmembrane Anion Exchangers in Sperm Post-testicular Maturation and Fertilization Potential

Solute carrier 26 (SLC26) members are conserved membranous proteins that mediate the transport of various anions across the plasma membrane of epithelial cells and constitute important regulators of pH and HCO3– concentration and contributions to ionic- and pH-dependent processes during sperm post-testicular maturation are focused on.

Spatio-temporal landscape of mouse epididymal cells and specific mitochondria-rich segments defined by large-scale single-cell RNA-seq

The single-cell transcriptome atlas of the mouse epididymis is presented and revealed the novel distribution pattern of mitochondria and key genes that may be linked to sperm functionalities in the first wave and subsequent wave of sperm, providing a roadmap to be emulated in efforts to achieve sperm maturation regulation in the epididsymis.

Network Analyses Predict Small RNAs That Might Modulate Gene Expression in the Testis and Epididymis of Bos indicus Bulls

It is suggested that bta-mir-2886 and other small RNAs might modulate gene expression in the testis and epididymis, in Bos indicus cattle.

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