Mapping the sites of localization of epithelial sodium channel (ENaC) and CFTR in segments of the mammalian epididymis

@article{Sharma2019MappingTS,
  title={Mapping the sites of localization of epithelial sodium channel (ENaC) and CFTR in segments of the mammalian epididymis},
  author={Sachin Sharma and Israel Hanukoglu},
  journal={Journal of Molecular Histology},
  year={2019},
  volume={50},
  pages={141-154}
}
The sperm produced in the seminiferous tubules pass through the rete testis, efferent ducts, and epididymis. The epididymis has three distinct regions known as caput, corpus, and cauda. The transit through the epididymis is an essential process in sperm maturation. The lumen of each epididymal region has a unique fluid composition regulated by many ion channels and transporters in the epithelial cells. The objective of this study was to map the sites of localization of ion channels ENaC and… Expand
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High‐resolution imaging of the actin cytoskeleton and epithelial sodium channel, CFTR, and aquaporin‐9 localization in the vas deferens
TLDR
The localization of all three channels on the apical surface of the columnar epithelial cells provides clear evidence that these channels are involved concurrently in the regulation of fluid and electrolyte balance in the lumen of the vas deferens. Expand
An atlas of human proximal epididymis reveals cell-specific functions and distinct roles for CFTR
TLDR
A single cell atlas of the human proximal epididymis reveals key cell-specific functions, regional specialization, and two roles for CFTR, which is most abundant in surface epithelial cells in the efferent ducts and in rare clear Cells in the caput epidIDymis, suggesting region-specific functional properties. Expand
Identification and classification of epithelial cells in nephron segments by actin cytoskeleton patterns
TLDR
This study highlights the power of high‐resolution confocal microscopy for identifying specific cell types using the simple probe of F‐actin‐binding phalloidin to identify whole kidney sections by confocal microscopic imaging of fluorescent phalloids. Expand
Development and Differentiation of Epididymal Epithelial Cells in Korean Native Black Goat
TLDR
This study is the first to use immunofluorescence labeling and confocal imaging to characterize the differentiation of clear cells and basal cells in the goat epididymis, and could help the understanding of the regulatory mechanism that creates an optimal luminal environment for sperm maturation and storage in the epididykis. Expand
Epithelial sodium channel (ENaC) (version 2019.4) in the IUPHAR/BPS Guide to Pharmacology Database
The epithelial sodium channels (ENaC) are located on the apical membrane of epithelial cells in the distal kidney tubules, lung, respiratory tract, male and female reproductive tracts, sweat and… Expand
The Distribution and Role of the CFTR Protein in the Intracellular Compartments
TLDR
A summary of the present state of knowledge regarding CFTR localization and function in intracellular compartments, the physiological role of this localization and the consequences of protein dysfunction at cellular, epithelial and organ levels are provided. Expand
Importance of SLC26 Transmembrane Anion Exchangers in Sperm Post-testicular Maturation and Fertilization Potential
  • A. Touré
  • Medicine, Chemistry
  • Front. Cell Dev. Biol.
  • 2019
TLDR
Solute carrier 26 (SLC26) members are conserved membranous proteins that mediate the transport of various anions across the plasma membrane of epithelial cells and constitute important regulators of pH and HCO3– concentration and contributions to ionic- and pH-dependent processes during sperm post-testicular maturation are focused on. Expand
Spatio-temporal landscape of mouse epididymal cells and specific mitochondria-rich segments defined by large-scale single-cell RNA-seq
TLDR
The single-cell transcriptome atlas of the mouse epididymis is presented and revealed the novel distribution pattern of mitochondria and key genes that may be linked to sperm functionalities in the first wave and subsequent wave of sperm, providing a roadmap to be emulated in efforts to achieve sperm maturation regulation in the epididsymis. Expand
Network Analyses Predict Small RNAs That Might Modulate Gene Expression in the Testis and Epididymis of Bos indicus Bulls
TLDR
It is suggested that bta-mir-2886 and other small RNAs might modulate gene expression in the testis and epididymis, in Bos indicus cattle. Expand

References

SHOWING 1-10 OF 69 REFERENCES
Localization of epithelial sodium channel (ENaC) and CFTR in the germinal epithelium of the testis, Sertoli cells, and spermatozoa
TLDR
It is suggested that ENaC subunits in cytoplasmic pools in germ cells serve as the source of ENac subunits located along the tail of spermatozoa, and CFTR may have an independent role in gene regulation within the nuclei. Expand
Epithelial sodium channels (ENaC) are uniformly distributed on motile cilia in the oviduct and the respiratory airways
TLDR
Findings indicate that ENaC functions as a regulator of the osmolarity of the periciliary fluid bathing the cilia, and requires reevaluation of the mechanisms of action of CFTR and other modulators of EN aC function. Expand
Expression of epithelial sodium channel (ENaC) and CFTR in the human epidermis and epidermal appendages
TLDR
The results reveal that ENaC is expressed strongly in all epidermal layers except stratum corneum, and also in the sebaceous glands, eccrine glands, arrector pili smooth muscle cells, and intra-dermal adipocytes, and co-localized with F-actin. Expand
Ovine male genital duct epithelial cells differentiate in vitro and express functional CFTR and ENaC.
TLDR
It is suggested that cultured ovine vas deferens and epididymis cells absorb Na(+) via amiloride-sensitive epithelial Na(+ channels (ENaC) and secrete Cl(-) and HCO(-)(3) via apical cystic fibrosis transmembrane conductance regulator (CFTR) Cl(+) channels. Expand
High-resolution helium ion microscopy of epididymal epithelial cells and their interaction with spermatozoa.
TLDR
HIM technology represents a major imaging breakthrough that can be successfully applied to study the epididymis and spermatozoa, with the goal of advancing the understanding of their structure and function. Expand
Polarized Distribution of NHE1 and NHE2 in the Rat Epididymis
TLDR
Results from the present study may suggest that the apical NHE2 is involved in Na+ reabsorption and the basolateral NHE1 in HCO3− secretion in the rat epididymis. Expand
Characteristics of the Epididymal Luminal Environment Responsible for Sperm Maturation and Storage
TLDR
Current understanding of the molecular mechanisms that exert influence over the unique intraluminal environment of the epididymis is reviewed, with a particular focus on vesicle-dependent mechanisms that facilitate intercellular communication between the epidIDymal soma and maturing sperm cell population. Expand
Ultrastructural and immunohistochemical studies of rat epididymis
TLDR
The heterogeneous appearance of the contractile cells of the wall seemed to support the different contractile pattern of the epididymal regions: caput, corpus and cauda, thus suggesting a contractile function of this cell type. Expand
Acquisition of volume regulatory response of sperm upon maturation in the epididymis and the role of the cytoplasmic droplet
TLDR
The literature on the fate of the sperm's cytoplasmic droplet is reviewed and a role for it in volume regulation is suggested, possibly driven by a mechanism of regulatory volume increase invoked by the hypertonicity of epididymal luminal fluid. Expand
Establishment of cell-cell cross talk in the epididymis: control of luminal acidification.
TLDR
This review describes how epididymal epithelial cells work in a concerted manner, together with spermatozoa, to establish and maintain this acidic luminal environment. Expand
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