Mapping the orientation of nuclear pore proteins in living cells with polarized fluorescence microscopy

@article{Kampmann2011MappingTO,
  title={Mapping the orientation of nuclear pore proteins in living cells with polarized fluorescence microscopy},
  author={M. Kampmann and C. E. Atkinson and Alexa L. Mattheyses and S. Simon},
  journal={Nature structural & molecular biology},
  year={2011},
  volume={18},
  pages={643 - 649}
}
  • M. Kampmann, C. E. Atkinson, +1 author S. Simon
  • Published 2011
  • Biology, Medicine
  • Nature structural & molecular biology
  • The nuclear pore complex (NPC) perforates the nuclear envelope to facilitate selective transport between nucleus and cytoplasm. The NPC is composed of multiple copies of ∼30 different proteins, termed nucleoporins, whose arrangement within the NPC is an important unsolved puzzle in structural biology. Various alternative models for NPC architecture have been proposed but not tested experimentally in intact NPCs. We present a method using polarized fluorescence microscopy to investigate… CONTINUE READING
    64 Citations

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