• Biology, Medicine
  • Published in
    Nature Structural &Molecular…
    2011
  • DOI:10.1038/nsmb.2056

Mapping the orientation of nuclear pore proteins in living cells with polarized fluorescence microscopy

@inproceedings{Kampmann2011MappingTO,
  title={Mapping the orientation of nuclear pore proteins in living cells with polarized fluorescence microscopy},
  author={Martin Kampmann and Claire E. Atkinson and Alexa L Mattheyses and Sanford M Simon},
  booktitle={Nature Structural &Molecular Biology},
  year={2011}
}
The nuclear pore complex (NPC) perforates the nuclear envelope to facilitate selective transport between nucleus and cytoplasm. The NPC is composed of multiple copies of ∼30 different proteins, termed nucleoporins, whose arrangement within the NPC is an important unsolved puzzle in structural biology. Various alternative models for NPC architecture have been proposed but not tested experimentally in intact NPCs. We present a method using polarized fluorescence microscopy to investigate… CONTINUE READING

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