Mapping the collagen-binding site of human fibronectin by expression in Escherichia coli.

@article{Owens1986MappingTC,
  title={Mapping the collagen-binding site of human fibronectin by expression in Escherichia coli.},
  author={Raymond J. Owens and Francisco E. Baralle},
  journal={The EMBO journal},
  year={1986},
  volume={5 11},
  pages={2825-30}
}
The collagen-binding domain of human fibronectin has been expressed as a cro/beta-galactosidase fusion protein in Escherichia coli. The hybrid polypeptide was recognized by an anti-(human plasma fibronectin) serum and bound specifically to gelatin-Sepharose. The collagen-binding region was subdivided by constructing a series of overlapping bacterial expression plasmids. The fusion proteins produced by these constructs were analysed for gelatin-binding activity. The results indicate that the… CONTINUE READING

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