Mapping the Limits of Substrate Specificity of the Adenylation Domain of TycA

@article{Villiers2009MappingTL,
  title={Mapping the Limits of Substrate Specificity of the Adenylation Domain of TycA},
  author={Benoit R M Villiers and Florian Hollfelder},
  journal={ChemBioChem},
  year={2009},
  volume={10}
}
The limits and potential of substrate promiscuity of the adenylation domain of tyrocidine synthetase 1 were systematically explored. Substrate acceptance is governed by hydrophobic effects (as shown by the correlation of kcat/KM and side‐chain log P), shape complementarity and steric exclusion. The quantification of these factors provides ground rules for understanding and possibly evolving substrate specificity in this class of enzymes. 
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