Mapping studies and genetic analysis of transfer genes of the multi-resistant IncHI2 plasmid, R478.

@article{Page1999MappingSA,
  title={Mapping studies and genetic analysis of transfer genes of the multi-resistant IncHI2 plasmid, R478.},
  author={Diana T Page and Karyn Whelan and Emer Colleran},
  journal={FEMS microbiology letters},
  year={1999},
  volume={179 1},
  pages={
          21-9
        }
}
A bank of transfer-defective Tn7 insertion mutants of the multi-resistant IncHI2 megaplasmid, R478, was generated. Complementation analysis of these mutations identified a large 144-kb transfer-associated region of R478. A 6.8-kb segment from the transfer region was sequenced. The precise locations of Tn7 insertion within four distinct R478::Tn7 transfer-defective mutants were mapped and each insertion was found to disrupt a specific open reading frame. These transfer-associated determinants of… CONTINUE READING