Mapping structural differences between 30S ribosomal subunit assembly intermediates

@article{Holmes2004MappingSD,
  title={Mapping structural differences between 30S ribosomal subunit assembly intermediates},
  author={Kristi L Holmes and Gloria M Culver},
  journal={Nature Structural &Molecular Biology},
  year={2004},
  volume={11},
  pages={179-186}
}
Under appropriate conditions, functional Escherichia coli 30S ribosomal subunits assemble in vitro from purified components. However, at low temperatures, assembly stalls, producing an intermediate (RI) that sediments at 21S and is composed of 16S ribosomal RNA (rRNA) and a subset of ribosomal proteins (r-proteins). Incubation of RI at elevated temperatures produces a particle, RI*, of similar composition but different sedimentation coefficient (26S). Once formed, RI* rapidly associates with… CONTINUE READING