Mapping proteinDNA interactions in vivo with formaldehyde: Evidence that histone H4 is retained on a highly transcribed gene

@article{Solomon1988MappingPI,
  title={Mapping proteinDNA interactions in vivo with formaldehyde: Evidence that histone H4 is retained on a highly transcribed gene},
  author={M. Solomon and P. L. Larsen and A. Varshavsky},
  journal={Cell},
  year={1988},
  volume={53},
  pages={937-947}
}
  • M. Solomon, P. L. Larsen, A. Varshavsky
  • Published 1988
  • Biology, Medicine
  • Cell
  • We have used formaldehyde-mediated protein-DNA crosslinking within intact cells to examine the in vivo chromatin structure of the D. melanogaster heat shock protein 70 (hsp70) genes. In agreement with previous in vitro studies, we find that the heat shock-mediated transcriptional induction of the hsp70 genes perturbs their chromatin structure, resulting in fewer protein-DNA contacts crosslinkable in vivo by formaldehyde. However, contrary to earlier in vitro evidence that histones may be absent… CONTINUE READING

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    Chromatin immunoprecipitation (ChIP).
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    References

    Publications referenced by this paper.
    SHOWING 1-10 OF 58 REFERENCES
    Chromatin fine structure of active and repressed genes
    • 91