Mapping protein-protein interactions for the yeast ABC transporter Ycf1p by integrated split-ubiquitin membrane yeast two-hybrid analysis.

@article{Paumi2007MappingPI,
  title={Mapping protein-protein interactions for the yeast ABC transporter Ycf1p by integrated split-ubiquitin membrane yeast two-hybrid analysis.},
  author={Christian M. Paumi and Javier Men{\'e}ndez and Anthony Arnoldo and Kim Engels and Kavitha Ravee Iyer and Safia Thaminy and Oleg G. Georgiev and Yves Barral and Susan Michaelis and Igor Stagljar},
  journal={Molecular cell},
  year={2007},
  volume={26 1},
  pages={15-25}
}
The ATP binding cassette (ABC) transporters are important in human health and disease and represent the largest family of transmembrane proteins; however, their highly hydrophobic nature complicates the use of standard biochemical approaches to identify interacting proteins. Here, we report the development of a modified version of the split-ubiquitin membrane yeast two-hybrid (MYTH) technology using genomically integrated "bait" constructs, hence the designation iMYTH. We used iMYTH in a… CONTINUE READING

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