Mapping oxygen accessibility to ribonuclease a using high-resolution NMR relaxation spectroscopy.

@article{Teng2004MappingOA,
  title={Mapping oxygen accessibility to ribonuclease a using high-resolution NMR relaxation spectroscopy.},
  author={Chui Ling Teng and Robert L. Bryant},
  journal={Biophysical journal},
  year={2004},
  volume={86 3},
  pages={
          1713-25
        }
}
Paramagnetic contributions to nuclear magnetic spin-lattice relaxation rate constant induced by freely diffusing molecular oxygen measured at hundreds of different protein proton sites provide a direct means for characterizing the exploration of the protein by oxygen. This report focuses on regions of ribonuclease A where the rate constant enhancements are either quite large or quite small. We find that there are several regions of enhanced oxygen affinity for the protein both on the surface… CONTINUE READING
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