Mapping of the normal human corneal sub-Basal nerve plexus by in vivo laser scanning confocal microscopy.

@article{Patel2005MappingOT,
  title={Mapping of the normal human corneal sub-Basal nerve plexus by in vivo laser scanning confocal microscopy.},
  author={Dipika V Patel and Charles N J McGhee},
  journal={Investigative ophthalmology \& visual science},
  year={2005},
  volume={46 12},
  pages={
          4485-8
        }
}
  • D. Patel, C. McGhee
  • Published 1 December 2005
  • Medicine, Biology
  • Investigative ophthalmology & visual science
PURPOSE To produce a two-dimensional reconstruction map of the living human sub-basal corneal nerve plexus using in vivo confocal microscopy. METHODS Laser scanning in vivo confocal microscopy was performed on three normal eyes of three healthy human subjects. Subjects were asked to fixate on targets arranged in a grid to enable examination of the cornea in a wide range of positions. Using the section mode, a mean of 573 +/- 176 images of the sub-basal plexus were obtained for each subject… 
View on PubMed
iovs.arvojournals.org
243 Citations
Highly Influential Citations
12
Background Citations
54
Methods Citations
23
Results Citations
7

243 Citations

Citation Type

Citation Type

In vivo laser scanning confocal microscopy confirms that the human corneal sub-basal nerve plexus is a highly dynamic structure.
TLDR
This study provides strong evidence that the living human sub-basal corneal nerve plexus is a highly dynamic structure, with continuous centripetal movement of identifiable branch points of up to 26 microm/wk, creating dramatic pattern changes in the plexi over a 6-week period.
Mapping the corneal sub-basal nerve plexus in keratoconus by in vivo laser scanning confocal microscopy.
TLDR
This is the first study to elucidate the overall distribution of sub-basal nerves in the living central to midperipheral human cornea in keratoconus, using laser scanning in vivo confocal microscopy.
Interocular Comparison by In Vivo Confocal Microscopy of the 2-Dimensional Architecture of the Normal Human Corneal Subbasal Nerve Plexus
TLDR
This study reveals the marked similarities between the corneal subbasal nerve plexus configuration in the right and left eyes of the living human cornea, highlighting that the typical mirror-image symmetry in cornesal topographic patterns is not obeyed in respect to corneals innervation and that a clockwise orientation of the sub Basal plexi is typically encountered.
Mapping of Normal Corneal K-Structures by In Vivo Laser Confocal Microscopy
TLDR
The hypothesis that the K-structures are the anterior collagen fiber bundles running at the posterior surface of the Bowman layer and thus are the structural basis for ACM formation is supported.
Real-time mapping of the corneal sub-basal nerve plexus by in vivo laser scanning confocal microscopy
TLDR
The developed method enables a real-time in vivo mapping of the sub-basal nerve plexus which is stringently necessary for statistically firmed conclusions about morphometric plexi alterations.
In vivo three-dimensional confocal laser scanning microscopy of the epithelial nerve structure in the human cornea
TLDR
3D-CLSM allows analysis of the spatial arrangement of the anterior corneal nerves and visualization of the epithelium and keratocytes in the living human cornea and provides a basis for further studies of alterations of the cellular arrangement and epithelial innervation in cornea disease.
Ex vivo confocal microscopy of human corneal nerves
TLDR
This study establishes a direct link between sub-basal nerves and the sub-Bowman's nerves via distinct terminal bulbs, which is seen in all quadrants and can be traced to the corneal centre.
Standardized baseline human corneal subbasal nerve density for clinical investigations with laser-scanning in vivo confocal microscopy.
TLDR
A baseline human corneal subbasal nerve density has been determined by laser-scanning in vivo confocal microscopy of the central cornea using rigorous methods, and the methods and results could aid in the future assessment ofCorneal nerves in various patient populations.
[On-line mapping of corneal structures with in vivo laser scanning microscopy].
TLDR
On-line mapping of cornea with in vivo confocal microscopy allows one to perform a large area 2D reconstruction and analyses of normal and pathological cornea and is considerably better than existing off-line reconstruction possibilities in terms of image quality and time consumption.
Real-time mapping of the subepithelial nerve plexus by in vivo confocal laser scanning microscopy
TLDR
The described method permits real-time in vivo mapping of the SEP, thus providing the necessary basis for statistically robust conclusions concerning morphometric plexus alterations.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 18 REFERENCES
In vivo human corneal confocal microscopy of identical fields of subepithelial nerve plexus, basal epithelial, and wing cells at different times
A technique is described to obtain time‐lapse reflected light confocal images of cells in the basal epithelium and adjacent wing cell layer from the in vivo human cornea. The technique is based on…
The Effect of Age on the Corneal Subbasal Nerve Plexus
TLDR
The density and orientation of the subbasal nerve plexus in the central human cornea does not change with age, as measured by tandem scanning confocal microscopy.
Morphology of Corneal Nerves Using Confocal Microscopy
TLDR
This study provides convincing evidence of the suitability of confocal microscopy to image corneal nerves, the only drawback being the limited resolution in terms of the differentiation of the ultrastructure of nerve bundles.
Architecture of human corneal nerves.
TLDR
The nerve bundles in the subbasal plexus of the human cornea form a regular dense meshwork with equal density over a large central and central-peripheral area.
Scanning slit confocal microscopic observation of cell morphology and movement within the normal human anterior cornea.
In vivo confocal microscopic evaluation of Langerhans cell density and distribution in the normal human corneal epithelium
TLDR
The Heidelberg Retina Tomograph II in combination with the Rostock Cornea Module enables in vivo assessment of density and distribution of LCs in the corneal epithelium, providing insight into human eye immunology.
Centripetal movement of corneal epithelial cells in the normal adult mouse.
TLDR
Corneal epithelium of adult GFP mice exhibits a pattern of GFP expression that is suitable for studying cell movement in the normal cornea, and clusters of several high-GFP cells were tracked in living mice for up to 7 weeks, and an analysis of time-lapse sequences revealed that they moved centripetally at an average rate of 26 micro m/d.
Ultrastructural organization of human corneal nerves.
TLDR
The presence of vesicles, mitochondria, and glycogen in stromal and epithelial nerve fibers suggest that classical and peptidergic transmitters, probably of sensory origin, innervate the human cornea.
Sheet preparations expose the dermal nerve plexus of human skin and render the dermal nerve end organ accessible to extensive analysis.
TLDR
A method based on the immuno-staining of dermal sheet preparations for subsequent analysis by electron-, light- or laser scanning microscopy that should allow the acquisition of new insights into the highly organized architecture of the skin nerve end organ.
Nerve structures in human central corneal epithelium
  • B. Schimmelpfennig
  • Biology, Medicine
    Graefe's Archive for Clinical and Experimental Ophthalmology
  • 2005
TLDR
The topography of epithelial nerves was examined by light microscopy in epithelial flat preparations dissected from the stroma and Bowman's membrane penetrating stromal nerves constituted the innervation of human central corneal epithelium.
...
1
2
...