Mapping of the RNA-binding domain of the cucumber mosaic virus movement protein.

@article{Vaquero1997MappingOT,
  title={Mapping of the RNA-binding domain of the cucumber mosaic virus movement protein.},
  author={Carmen G{\'o}mez Vaquero and Yu Cai Liao and J{\"o}rg M. N{\"a}hring and Ruediger Fischer},
  journal={The Journal of general virology},
  year={1997},
  volume={78 ( Pt 8)},
  pages={
          2095-9
        }
}
A series of in-frame deletion mutants was used to identify a domain within the 3a protein of cucumber mosaic virus (CMV) that is required for RNA-binding activity. Deletions in the 3a gene were generated by PCR and restriction digestion, and the resulting mutated 3a sequences were cloned either in pT7-7 or in pGEX-5X3 expression vectors. The mutated 3a proteins or fusions with glutathione S-transferase (GST) were expressed in E. coli, purified, and their nucleic acid-binding activities analysed… CONTINUE READING

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