Mapping of regions within the vaccinia virus complement control protein involved in dose-dependent binding to key complement components and heparin using surface plasmon resonance.

@article{Smith2003MappingOR,
  title={Mapping of regions within the vaccinia virus complement control protein involved in dose-dependent binding to key complement components and heparin using surface plasmon resonance.},
  author={Scott A. Smith and Rajini Sreenivasan and Gunasekaran Krishnasamy and Ken W Judge and Krishna H. M. Murthy and Shrihari J Arjunwadkar and D. R. Pugh and Girish J. Kotwal},
  journal={Biochimica et biophysica acta},
  year={2003},
  volume={1650 1-2},
  pages={30-9}
}
The vaccinia virus complement control protein (VCP) is involved in modulating the host inflammatory response by blocking both pathways of complement activity through its ability to bind C3b and C4b. Other activities arise from VCP's ability to strongly bind heparin. To map regions within VCP involved in binding complement and heparin experimentally, surface plasmon resonance (SPR) and recombinantly expressed VCP (rVCP) constructs were employed. Using C3b or heparin as the immobilized ligand… CONTINUE READING