Mapping of receptor binding sites on IL-1 beta by reconstruction of IL-1ra-like domains.

@article{Boraschi1995MappingOR,
  title={Mapping of receptor binding sites on IL-1 beta by reconstruction of IL-1ra-like domains.},
  author={Diana Boraschi and Paola Boss{\`u} and Paolo Ruggiero and Aldo Tagliabue and Riccardo Bertini and Gianfranco Macchia and C Gasbarro and Luca Pellegrini and Giovanni Melillo and E Ulisse and U Visconti and Cinzia Bizzarri and Erika Del Grosso and Andrew Reay Mackay and Gianni Frascotti and Francesca Frigerio and Renata M. Grifantini and Guido Grandi},
  journal={Journal of immunology},
  year={1995},
  volume={155 10},
  pages={4719-25}
}
Upon structure comparison between IL-1 beta and its antagonist IL-1ra, single or multiple residues along the IL-1 beta sequence were replaced with the corresponding amino acids present in the IL-1ra protein, in the attempt to identify sites important for receptor binding and for biologic activity on the two molecules. Ten of fifteen mutant proteins had… CONTINUE READING