Mapping of prolactin and tumor necrosis factor-beta genes on human chromosome 6p using lymphoblastoid cell deletion mutants

  title={Mapping of prolactin and tumor necrosis factor-beta genes on human chromosome 6p using lymphoblastoid cell deletion mutants},
  author={Alida M. Evans and Jean W. Petersen and Gurbax Singh Sekhon and Robert Demars},
  journal={Somatic Cell and Molecular Genetics},
A collection of human B lymphoblastoid cell lines (LCLs) was used to map two genetic sequences for which polymorphism had not been identified: human prolactin (PRL) and tumor necrosis factor-beta (TNFB). The LCLs have overlapping deletions on chromosome 6p produced by gamma-irradiation of LCL 721. After using two chromosome 6p sequences for which LCL 721 is heterozygous to validate our scanning denistometry (SD) method for inferring gene copy number, SD was used to map TNFBand PRL. TNFBmaps to… 

Characterization of a prolactin gene polymorphism and its associations with systemic lupus erythematosus.

A functionally significant polymorphism is found that alters prolactin promoter activity and mRNA levels in the lymphocytes that may contribute to disease progression by affecting T cell function.

Polymorphisms of the human prolactin gene—implications for production of lymphocyte prolactin and systemic lupus erythematosus

Although prolactin is likely to be one of several predisposing factors in the pathogenesis and progression of SLE, this suggests that manipulation of lymphocyte Prolactin production (rather than pituitary production) might be a useful therapeutic approach.

Molecular characterization and an 80-bp indel polymorphism within the prolactin receptor (PRLR) gene and its associations with chicken growth and carcass traits

An 80-bp insertion/deletion (indel) polymorphism in the 3′-untranslated region (3′-UTR) of the chicken PRLR gene has potential as a new molecular marker for marker-assisted selection of chicken growth and carcass traits.

Prolactin Promoter Polymorphism (−1149 G/T) is Associated with ANTI–DNA Antibodies in Mexican Patients with Systemic Lupus Erythematosus

The data demonstrate that the prolactin promoter polymorphism −1149 G/T does not significantly contribute to SLE disease susceptibility but does predispose carriers to other immunological changes.

The Bg/II polymorphism of the human prolactin gene lies within intron C and can be detected by PCR/RFLP.

  • F. R. StevensA. Hajeer W. Ollier
  • Biology
    European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics
  • 1999
A PCR-RFLP method to genotype individuals is developed and a Bg/II polymorphism is mapped to intron C and the base mutation that causes it is detected.

Big Prolactin 60 kDa is Overexpressed in Salivary Glandular Epithelial Cells from Patients with Sjögren's Syndrome

PRL-like proteins are synthetized and overexpressed in glandular epithelial cells of labial salivary glands from SS patients and correlate with the aggressiveness of the disease.

Macroprolactin: searching for a needle in a haystack?

A number of physiological and pathological conditions have been associated with macroprolactinemia, including stress, pregnancy, strenuous physical exercise, pituitary adenomas and cancers, mechanical stimulation of breast, chest wall trauma, hepatorenal disease, primary hypothyroidism, intracranial tumors compressing pituitaries, empty sella syndrome, treatment with prolactin stimulating drugs and autoimmune disorders.

Polymorphisms in hormone metabolism and growth factor genes and mammographic density in Norwegian postmenopausal hormone therapy users and non-users

Some evidence is found that variants in the PRL gene were associated with MD in current EPT and NETA users, which may suggest that several genes in the hormone metabolism and growth factor pathways are implicated in determining MD.

Functional –1149 G/T Polymorphism of the Prolactin Gene in Schizophrenia

A possible abnormality of the functional –1149 G/T polymorphism of the prolactin gene in schizophrenia, especially in male patients, similar to that found in autoimmune diseases such as systemic lupus erythematosus and rheumatoid arthritis could also correspond with an autoimmune pathogenesis of schizophrenia.



Differential implication of deoxyribonucleic acid methylation in rat prolactin and rat growth hormone gene expressions: a comparison between rat pituitary cell strains.

Analysis of cleavage patterns generated by the isoschizomeric restriction enzymes HpaII and MspI in DNA isolated from rat pituitary cell lines showed that other events are required for the full expression of rPRL and rGH genes in addition to their own demethylation, suggesting a variable importance of gene methylation.

Isolation and characterization of the human prolactin gene.

The hPRL gene is present as a single copy per haploid genome, is approximately 10 kb long and contains four introns, three of which interrupt the coding sequence at the same locations as in the known GH and PRL genes.

Localization of the human MHC-linked complement genes between HLA-B and HLA-DR by using HLA mutant cell lines.

A C4 cDNA clone has been used to localize the C4 genes within the human major histocompatibility complex (MHC) and permitted the mapping of the C 4 genes, and the other MHC-linked complement genes, between the HLA-DR and Hla-B loci.

Construction of a map of the short arm of human chromosome 6.

A genetic map of the short arm of chromosome 6 has been constructed on the basis of linkage studies with the eight markers and will be useful in the study of HLA-associated diseases.

SB: a new HLA-linked human histocompatibility gene defined using HLA-mutant cell lines

LCL-721 mutants are characterized for expression of both HLA-DR and SB, and five new secondary B-cell antigens have recently been defined, which evoke strong secondary allogeneic proliferative and cytotoxic responses.

Use of HLA loss mutants to analyse the structure of the human major histocompatibility complex

One cDNA clone of an HLA class I gene, pHLA-1, in combination with γ-ray-induced HLA loss variants, will be used to identify recombinant DNA HLA clones and to correlate individual DNA restriction fragments with expression of specific HLA alleles.

Gamma ray-induced loss of expression of HLA and glyoxalase I alleles in lymphoblastoid cells.

Gamma rays from a cesium source were used to generate human lymphoblastoid cell line variants that had lost expression of all major histocompatibility complex antigens coded for by a single haplotype, showing that expressions of HLA and GLO alleles trans to B8 were retained.

The prolactin gene is located on chromosome 6 in humans.

The gene for prolactin has been located on chromosome 6 in humans in a different location from those of the genes for the related hormones chorionic somatomammotropin and growth hormone, and in somatic cell hybrids of human and mouse cells the 7.4-, 3.6-, and 3.3-kilobase mouse fragments were always present.

Linkage map of the human major histocompatibility complex including the tumor necrosis factor genes.

A restriction map of the entire human major histocompatibility complex of about 3500 kb has been prepared and the TNF genes are located 200 kilobases of HLA-B and about 350 kb telomeric of the class III cluster.