Mapping of prolactin and tumor necrosis factor-beta genes on human chromosome 6p using lymphoblastoid cell deletion mutants

@article{Evans1989MappingOP,
  title={Mapping of prolactin and tumor necrosis factor-beta genes on human chromosome 6p using lymphoblastoid cell deletion mutants},
  author={Alida M. Evans and Jean W. Petersen and Gurbax Singh Sekhon and Robert Demars},
  journal={Somatic Cell and Molecular Genetics},
  year={1989},
  volume={15},
  pages={203-213}
}
A collection of human B lymphoblastoid cell lines (LCLs) was used to map two genetic sequences for which polymorphism had not been identified: human prolactin (PRL) and tumor necrosis factor-beta (TNFB). The LCLs have overlapping deletions on chromosome 6p produced by gamma-irradiation of LCL 721. After using two chromosome 6p sequences for which LCL 721 is heterozygous to validate our scanning denistometry (SD) method for inferring gene copy number, SD was used to map TNFBand PRL. TNFBmaps to… 

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  • F. R. StevensA. Hajeer W. Ollier
  • Biology
    European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics
  • 1999
A PCR-RFLP method to genotype individuals is developed and a Bg/II polymorphism is mapped to intron C and the base mutation that causes it is detected.

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