Mapping of intra-locus duplications and introgressed DNA: aids to map-based cloning of genes from complex genomes illustrated by physical analysis of the Rx locus in tetraploid potato

@article{Kanyuka1999MappingOI,
  title={Mapping of intra-locus duplications and introgressed DNA: aids to map-based cloning of genes from complex genomes illustrated by physical analysis of the Rx locus in tetraploid potato},
  author={Kostya Kanyuka and Abdel Bendahmane and Jeroen N. A. M. Rouppe van der Voort and Edwin van der Vossen and David C. Baulcombe},
  journal={Theoretical and Applied Genetics},
  year={1999},
  volume={98},
  pages={679-689}
}
 We describe here novel approaches to high-resolution mapping of repeated and introgressed DNA which may prove generally useful in map-based cloning from complex genomes. These approaches were developed in order to clone the Rx locus in potato. First, we prepared a BAC library from a tetraploid plant carrying Rx in the duplex condition (Rx, Rx, rx, rx). BAC clones were then isolated with close markers on either side of Rx. However these clones did not extend across Rx: in the cloned DNA the… CONTINUE READING
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