Mapping of human thyroglobulin gene on the long arm of chromosome 8 by in situ hybridization

  title={Mapping of human thyroglobulin gene on the long arm of chromosome 8 by in situ hybridization},
  author={Vittorio Enrico Avvedimento and Roberto Di Lauro and Antonella Monticelli and Francesco Bernardi and P. Patracchini and Elisa Calzolari and Giuseppe Martini and Stelio Varrone},
  journal={Human Genetics},
SummaryWe report the structural organization of a segment of the human thyroglobulin gene, located 70kb from the 3′ end of the gene, containing the exons 8 and 9 starting from the 3′ end. Selected probes from this region have been used for the chromosomal mapping of the thyroglobulin gene by in situ hybridization techniques. Only one site in the human haploid karyotype is labeled with the genomic DNA probes. Twenty percent of the grains are localized on the long arm of chromosome 8, mostly in… 
Molecular advances in thyroglobulin disorders.
  • C. Rivolta, H. Targovnik
  • Biology, Medicine
    Clinica chimica acta; international journal of clinical chemistry
  • 2006
Gene mapping and medical genetics Human chromosome 8
The 27 genes assigned to chromosome 8 at the Ninth Human Gene Mapping Workshop thus represent a third of the expected number, and in six loci corresponding to fragile sites, three pseudogenes, and four gene-like sequences were is a of the 500 to 5000 gene loci expected from a genome that 10 000 and 100 000.
Integrity of the thyroglobulin locus in tricho-rhino-phalangeal syndrome II
The results indicate that the locus of the thyroglobulin gene is intact in the patient with an interstitial deletion of proximal band 8q24, which confirms its more distal localization reported earlier by Bergé-Lefranc et al. (1985).
De novo complex autosomal translocation involving chromosomes 8, 13 and 15 in a girl with a sporadic retinoblastoma
A 5-month-old female with sporadic monolateral retinoblastoma with a constitutional de novo complex autosomal translocation involving chromosomes 8, 13 and 15 resulting in a deletion of chromosome 13q14 confirmed by esterase D assay is reported.
enetics and phenomics of hypothyroidism and goiter due to thyroglobulin utations
Dyshormonogenesis displays a wide phenotype variation and is characterized usually by the presence of congenital goiter or goiter appearing shortly after birth, high 131I uptake, negative perchlorate discharge test, low serum TG and elevated serum TSH with simultaneous low serum T4 and low, normal or high serum T3.


The human thyroglobulin gene contains two 15-17 kb introns near its 3'-end.
Cloned overlapping segments of the human thyroglobulin gene are cloned from a genomic cosmid library and blot hybridization under non-stringent conditions shows the presence of only one copy of this gene in the human genome and the absence of other closely related sequences.
Localization of the human insulin gene to the distal end of the short arm of chromosome 11.
The results demonstrate chromosomal localization of the human insulin gene to 11p15 and a significant percentage of hybridized cells exhibited silver grains on the distal end of the short arm (band p15) of chromosome 11.
Structural organization of the 5' region of the human thyroglobulin gene.
The availability of clones covering the region upstream from the thyroglobulin gene will provide the basis for the identification of sequences involved in its transcriptional control by thyroid-stimulating hormone (thyrotropin).
Structural organization of the 3' half of the rat thyroglobulin gene.
The structural organization of an 80 Kb segment of rat DNA, which encodes for about 40% of Thyroglobulin mRNA at the 3' end, is reported, and the minimum length of the rat TGB gene will be 150 Kb, which makes this gene the largest so far identified eukaryotic gene.
Localization of single copy DNA sequences on G-banded human chromosomes by in situ hybridization
In situ hybridization to human metaphase chromosomes of the 3H-labeled cloned DNA resulted in highly significant labeling of band p36 of chromosome 1, such that 22% of all chromosome grains were located on this region.
Chromosomal localization of a single copy gene by in situ hybridization ‐ human β globin genes on the short arm of chromosome 11
The localization of the β globin genes by in situ hybridization to fixed chromosomes is described and the results obtained imply that the polypeptide-like structure of these genes is determined by the H2O/O2 “spatially homologue” as well as by the “gene-by-gene” relationship as in 1.1.
Molecular Characteristics of a Non‐deletion α‐Thalassaemia of the Po River Delta
The form of α-thalassaemia of the Po river delta presents haematological and globin biosynthetic characteristics similar to α-halassaemic-1 but never gives rise to Hb H disease nor to hydrops foetalis, and is of the non-deletion type.
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
  • P. Thomas
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1980
A simple and rapid method for transferring RNA from agarose gels to nitrocellulose paper for blot hybridization has been developed, allowing removal of the hybridized probes and rehybridization of the RNA blots without loss of sensitivity.