Mapping of gene-specific markers on the genetic map of chickpea ( Cicer arietinum L.)

  title={Mapping of gene-specific markers on the genetic map of chickpea ( Cicer arietinum L.)},
  author={T. Pfaff and G{\"u}nter Kahl},
  journal={Molecular Genetics and Genomics},
  • T. Pfaff, G. Kahl
  • Published 12 March 2003
  • Biology, Medicine
  • Molecular Genetics and Genomics
With the exception of the fact that it is made up of eight different chromosomes, the physical organization of the 738-Mb genome of the important legume crop chickpea (Cicer arietinum L.) is unknown. In an attempt to increase our knowledge of the basic structure of this genome, we determined the map positions of a series of genes involved in plant defence responses (DR) by genetic linkage analysis. Exploiting the sequence data available in GenBank, we selected genes known to be induced in… 
A consensus genetic map of chickpea (Cicer arietinum L.) based on 10 mapping populations
A consensus genetic map of chickpea was constructed by merging linkage maps from 10 different populations, using STMS (Sequence-tagged Microsatellite Sites) as bridging markers, to assist breeders for selecting suitable markers to be used in marker-assisted selection (MAS).
Functional mapping in pea, as an aid to the candidate gene selection and for investigating synteny with the model legume Medicago truncatula
This paper reports the development of 63 single nucleotide polymorphism markers and 15 single-stranded conformation polymorphists for genes encoding enzymes mainly involved in primary metabolism, and their genetic mapping on a composite map using two pea recombinant inbred line populations and reports the genetic mapping of 21 of them in Medicago truncatula.
Development of microsatellite markers and analysis of intraspecific genetic variability in chickpea (Cicer arietinum L.)
The UPGMA based dendrogram was able to distinguish all the accessions except two accessions from Afghanistan establishing that microsatellites could successfully detect intraspecific genetic diversity in chickpea, and the variable numbers of AG repeats in different alleles were the major source of polymorphism.
Advances in Chickpea Genomics
Efforts have already begun on application of these genomics resources in chickpea improvement, and a more than 30 genetic linkage maps available in this crop are useful resources for genetic analysis and marker assisted breeding.
Development of ESTs from chickpea roots and their use in diversity analysis of the Cicer genus
Gene-based markers have proven to be effective tools for diversity analysis in Cicer and EST diversity analysis may be useful in identifying promising candidates for interspecific hybridization programs.
Molecular Mapping and Tagging of Fusarium Wilt Resistance in Chickpea (Cicer arietinum L.)
Analysis of variance for productive traits and wilt reaction at two stages indicated significant variation for all traits except number of branches among the RILs of both crosses.
Development and use of genic molecular markers (GMMs) for construction of a transcript map of chickpea (Cicer arietinum L.)
This is the first report on the development of large-scale genic markers including development of easily assayable markers and a transcript map of chickpea, and these resources should be useful not only for genome analysis and genetics and breeding applications of chick pea, but also for comparative legume genomics.
Development of SSR and gene-targeted markers for construction of a framework linkage map of Catharanthus roseus.
For the first time, a large array of STMS markers and GTMs was generated in the model medicinal plant C. roseus and will serve as a foundation for future genomics studies related to quantitative trait loci analysis and molecular breeding in C.roseus.
Identification of QTLS and Genes for Drought Tolerance Using Linkage Mapping and Association Mapping Approaches in Chickpea (Cicer arietinum)
Low levels of polymorphism and lack of sufficient numbers of molecular markers such as microsatellite or simple sequence repeats (SSRs) are the main constraints in chickpea improvement. Hence to
Advancing the STMS genomic resources for defining new locations on the intraspecific genetic linkage map of chickpea (Cicer arietinum L.)
This study reveals improvement of marker density and saturation of the region in the vicinity of sfl (double-podding) gene thereby bringing about an advancement of the current map of chickpea.


Construction of a Chickpea Linkage Map and Its Comparison With Maps of Pea and Lentil
An integrated genetic linkage map of chickpea (Clcei) has been developed that consists of 9 morphological, 27 isozyme, 10 RFLP, and 45 RAPD markers covering 550 cM, indicating considerable divergence of these genomes at the sequence level.
A linkage map of the chickpea (Cicer arietinum L.) genome based on recombinant inbred lines from a C. arietinum×C. reticulatum cross: localization of resistance genes for fusarium wilt races 4 and 5
The integrated molecular marker map of the chickpea genome was established using 130 recombinant inbred lines from a wide cross between a cultivar resistant to fusarium wilt caused by Fusarium oxysporum Schlecht to serve as a basis for marker-assisted selection and map-based cloning of fusaria wilt resistance genes and other agronomically important genes in future.
Characterization and mapping of sequence-tagged microsatellite sites in the chickpea (Cicer arietinum L.) genome
The present STMS marker map represents the most advanced co-dominant DNA marker map of the chickpea genome and was genetically mapped in 90 recombinant inbred lines from an inter-species cross between C. reticulatum and C. echinospermum.
Inheritance and linkage of a gene for resistance to race 4 of fusarium wilt and RAPD markers in chickpea
Results indicated that resistance is controlled by a single recessive gene in Fusarium oxysporum Schlechtend and the genes for resistance to race 1 and 4 are 5 map units apart.
The development of codominant PCR/RFLP based markers for the flax rust-resistance alleles at the L locus
The application of PCR markers to a set of 22 cultivars verifies genetic studies done by previous workers and demonstrates the usefulness of the markers for following segregation of L alleles in crosses amongst wide or narrow selections of cultivars.
Use of molecular markers in breeding for oligogenic disease resistance
The current and potential uses of molecular markers in breeding for oligogenic resistance traits and advantages of marker-facilitated selection for resistance genes under a variety of special circumstances are discussed.
Development of S-SAP markers based on an LTR-like sequence from Medicago sativa L.
Application of the Tms1 LTR-based S-SAP to double-stranded cDNA resulted in a complex banding pattern, demonstrating the presence of Tms2 LTRs within exons, and should prove suitable for studying genetic diversity within, and relatedness between, alfalfa species.
Sequence-tagged microsatellite site markers for chickpea (Cicer arietinum L.).
Two small-insert genomic libraries of chickpea were screened with a set of microsatellite-specific oligonucleotide probes and Mendelian inheritance of CaSTMS markers was demonstrated using aSet of recombinant inbred lines and their parents.
Inheritance and linkage relationships of morphological and isozyme loci in chickpea (Cicer arietinum L.)
Results suggest that pea, lentil and chickpea have several common linkage groups consisting of homologous genes, and indicates that linkages found in one genus can be used to predict similar linkages in related genera in the development of linkage maps.
Genetic relationships among annual species of Cicer(Fabaceae) using isozyme variation
The results suggest that the annual habit arose from perennial progenitors at least twice in the genus Cicer, which has a very distinct isozyme profile and peculiar morphological features.