Mapping of conformational mAb epitopes to the C domain of human angiotensin I-converting enzyme.

@article{Naperova2008MappingOC,
  title={Mapping of conformational mAb epitopes to the C domain of human angiotensin I-converting enzyme.},
  author={Irina A Naperova and Irina V. Balyasnikova and David E. Schwartz and Jean M. Watermeyer and Edward D. Sturrock and Olga A. Kost and Sergei M Danilov},
  journal={Journal of proteome research},
  year={2008},
  volume={7 8},
  pages={3396-411}
}
Angiotensin I-converting enzyme (ACE, CD143) has two homologous domains, each having a functional active site. Fine epitope mapping of 8 mAbs to the C-terminal domain of human ACE was carried out using plate precipitation assays, mAbs' cross-reactivity with ACE from different species, site-directed mutagenesis, and antigen- and cell-based ELISAs. Almost all… CONTINUE READING