Mapping of a new MAP kinase activated protein kinase gene (3PK) to human chromosome band 3p21.2 and ordering of 3PK and two cosmid markers in the 3p22–p21 tumour-suppressor region by two-colour fluorescencein situ hybridization

  title={Mapping of a new MAP kinase activated protein kinase gene (3PK) to human chromosome band 3p21.2 and ordering of 3PK and two cosmid markers in the 3p22–p21 tumour-suppressor region by two-colour fluorescencein situ hybridization},
  author={Anna Szeles and Svetlana Bajalica-Lagercrantz and Annika Lindblom and Tamara Lushnikova and Vladimir Kashuba and Stephan Imreh and Magnus Nordenskj{\"o}ld and George Klein and R. Zabarovsky},
  journal={Chromosome Research},
ANotl-linking clone NL1-210 (D3S1656) that contains the human MAP kinase activated protein kinase (3PK) gene was localized to 3p21.2 on DAPI-banded and propidium iodide (R-bands)-stained chromosomes by fluorescencein situ hybridization (FISH). For more precise localization of 3PK, two cosmid probes were used as a frame. In order to establish this frame, twoNotl-linking clones, NL2-008 (D3S1648) and NL3-003 (D3S3872) were used to screen the cosmid library for locus extension. They mapped to 3p21… 
Culture and expansion of the human embryonic stem cell line HS181, evaluated in a double-color system.
A two-color system allows analysis of colony formation and also helps to identify and follow the differentiation of cells and indicates that fusion events are extremely rare, or that after fusion the dual expression of both EGFP and RFP is not easily detected.
Cancer Progression Mediated by Horizontal Gene Transfer in an In Vivo Model
The results support the fact that cancer cells emit into the circulation biologically active DNA to foster tumor progression, and demonstrate that horizontal cancer progression mediated by circulating DNA occurs via its uptake by recipient cells.


3pK, a new mitogen-activated protein kinase-activated protein kinase located in the small cell lung cancer tumor suppressor gene region.
It is demonstrated that the new gene, referred to as 3pK (for chromosome 3p kinase), in fact encodes a mitogen-activated protein kinase-regulated protein serine-threonine kinase with a novel substrate specificity.
Alu-PCR approach to isolating NotI-linking clones from the 3p14-p21 region frequently deleted in renal cell carcinoma.
A modification of Alu-PCR as an approach to isolating NotI sites (e.g., CpG islands) from defined regions of the chromosome to isolate NotI-linking clones, which are natural markers on the chromosome, rather than random genomic fragments.
Nonrandom loss of human chromosome 3 fragments from mouse‐human microcell hybrids following progressive growth in SCID mice
These findings may be related to the postulated presence of tumor suppressor genes in the 3p24‐p21 region as indicated by the frequent deletion of this region in renal and small cell lung carcinomas and other solid tumors.
Common regions of deletion in chromosome regions 3p12 and 3p14.2 in primary clear cell renal carcinomas.
Results suggested that most RCCs exhibit loss in a region which brackets the t(3;8) familial chromosome translocation at 3p14.2, and some show additional deletions within the U2020 small cell lung carcinoma deletion at3p12.
Three distinct regions involved in 3p deletion in human lung cancer.
It is shown here that three distinct regions on 3p appear to be frequently deleted in lung cancer, including 3p25, 3p21.3 and 3p14-cen.
Isolation and mapping of polymorphic cosmid clones used for sublocalization of the multiple endocrine neoplasia type 1 (MEN1) locus
In order to localize the MEN1 gene further and to make its isolation possible, a number of new markers were isolated and used to sublocalize meiotic cross-overs more precisely in two MEN1 families, thus refining the mapping of the disease gene.
Construction of a human chromosome 3 specific NotI linking library using a novel cloning procedure.
Two new diphasmid vectors (lambda SK17 and SK22) and a novel procedure to construct linking libraries are described, which provide counter-selection against false linking clones in the library, and obviates the need for supF selection.
Loss of heterozygosity of chromosome 3p markers in small-cell lung cancer
The data show loss of alleles of chromosome 3p markers in tumour DNA of all nine patients supporting the hypothesis that this region contributes to tumorigenesis in SCLC.
Somatic cell hybrid panel and NotI linking clones for physical mapping of human chromosome 3.
The somatic cell hybrid panel and the regionally localized NotI linking probes should facilitate the construction of genetic linkage and physical maps to identify various tumor-suppressor and disease-related genes not only on the chromosome 3p, but on the entire chromosome.