Mapping nonrecombining regions in barley using multicolor FISH

Abstract

Fluorescence in situ hybridization (FISH) is a widely used method to localize DNA sequences on chromosomes. Out of the many uses, FISH facilitates construction of physical maps by ordering contigs of large-insert DNA clones, typically bacterial artificial chromosome (BAC) and establishing their orientation. This is important in genomic regions with low… (More)
DOI: 10.1007/s10577-013-9380-x

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