Mapping multiple co-sequenced T-DNA integration sites within the Arabidopsis genome

@article{Presting2003MappingMC,
  title={Mapping multiple co-sequenced T-DNA integration sites within the Arabidopsis genome},
  author={Gernot G. Presting},
  journal={Bioinformatics},
  year={2003},
  volume={19 5},
  pages={579-86}
}
MOTIVATION Insertion mutagenesis, using transgenes or endogenous transposons, is a popular method for generating null mutations (knockouts) in model organisms. Insertions are mapped to specific genes by amplifying (via TAIL-PCR) and sequencing genomic regions flanking the inserted DNA. The presence of multiple TAIL-PCR templates in one sequencing reaction… CONTINUE READING