Mapping initiation sites of DNA replication in vivo using polymerase chain reaction amplification of nascent strand segments.

Abstract

We describe a sensitive method for mapping replication initiation sites near regions of sequenced genomic DNA in vivo. It is based on selective amplification of sets of segments in purified nascent DNA strands and subsequent determination of the lengths of these strands required to include each member of the set. We demonstrate the ability of this method to… (More)

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