Mapping in vivo protein-RNA interactions at single-nucleotide resolution from HITS-CLIP data


Mammalian RNA complexity is regulated through interactions of RNA-binding proteins (RBPs) with their target transcripts. High-throughput sequencing together with UV-crosslinking and immunoprecipitation (HITS-CLIP) is able to globally map RBP-binding footprint regions at a resolution of ∼30-60 nucleotides. Here we describe a systematic way to analyze HITS… (More)
DOI: 10.1038/nbt.1873


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