Mapping in vivo protein-RNA interactions at single-nucleotide resolution from HITS-CLIP data

@article{Zhang2011MappingIV,
  title={Mapping in vivo protein-RNA interactions at single-nucleotide resolution from HITS-CLIP data},
  author={Chaolin Zhang and Robert B Darnell},
  journal={Nature Biotechnology},
  year={2011},
  volume={29},
  pages={607-614}
}
Mammalian RNA complexity is regulated through interactions of RNA-binding proteins (RBPs) with their target transcripts. High-throughput sequencing together with UV-crosslinking and immunoprecipitation (HITS-CLIP) is able to globally map RBP-binding footprint regions at a resolution of ∼30-60 nucleotides. Here we describe a systematic way to analyze HITS-CLIP data to identify exact crosslink sites, and thereby determine protein-RNA interactions at single-nucleotide resolution. We found that… CONTINUE READING
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