Mapping heterogeneity in patient-derived melanoma cultures by single-cell RNA-seq

  title={Mapping heterogeneity in patient-derived melanoma cultures by single-cell RNA-seq},
  author={Tobias Gerber and Edith Willscher and Henry Loeffler-Wirth and Lydia Hopp and Dirk Schadendorf and Manfred Schartl and Ulf Anderegg and Gray Camp and Barbara Treutlein and Hans Binder and Manfred Kunz},
Recent technological advances in single-cell genomics make it possible to analyze cellular heterogeneity of tumor samples. Here, we applied single-cell RNA-seq to measure the transcriptomes of 307 single cells cultured from three biopsies of three different patients with a BRAF/NRAS wild type, BRAF mutant/NRAS wild type and BRAF wild type/NRAS mutant melanoma metastasis, respectively. Analysis based on self-organizing maps identified sub-populations defined by multiple gene expression modules… CONTINUE READING
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