Mapping functional domains of colicin M.

  title={Mapping functional domains of colicin M.},
  author={Stephanie Helbig and Volkmar Braun},
  journal={Journal of bacteriology},
  volume={193 4},
Colicin M (Cma) lyses Escherichia coli cells by inhibiting murein biosynthesis through hydrolysis of the phosphate ester between C(55)-polyisoprenol and N-acetylmuramyl (MurNAc)-pentapeptide-GlcNAc in the periplasm. To identify Cma functional domains, we isolated 54 point mutants and small deletion mutants and examined their cytotoxicity levels. Activity and uptake mutants were distinguished by osmotic shock, which transfers Cma into the periplasm independent of the specific FhuA receptor and… CONTINUE READING
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