Mapping and sequence of the gene for the pseudorabies virus glycoprotein which accumulates in the medium of infected cells.

@article{Rea1985MappingAS,
  title={Mapping and sequence of the gene for the pseudorabies virus glycoprotein which accumulates in the medium of infected cells.},
  author={Thomas J. Rea and J G Timmins and Gary W. Long and L E Post},
  journal={Journal of virology},
  year={1985},
  volume={54 1},
  pages={21-9}
}
RNA from pseudorabies virus (PRV)-infected cells was translated in a reticulocyte lysate with and without the addition of dog pancreas microsomes. Upon addition of the microsomes to the translation reaction, an additional prominent protein product was observed that was not present when microsomes were omitted. The gene coding for this processed protein and its lower-molecular-weight precursor was mapped within the small unique region of the genome by hybridization of mRNA to cloned fragments of… CONTINUE READING

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